Overview
Type: Synthetic peptide
Form: Lyophilized powder
GLUT4 Blocking Peptide (#BLP-GT024) is the original antigen used for immunization during Anti-GLUT4 Antibody (#AGT-024) generation. The blocking peptide binds and ‘blocks’ Anti-GLUT4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) heart membranes:1,2. Anti-GLUT4 Antibody (#AGT-024), (1:400).
3,4. Anti-GLUT4 Antibody, preincubated with GLUT4 Blocking Peptide (#BLP-GT024).
Expression of Glucose Transporter 4 in rat olfactory bulbImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-GLUT4 Antibody (#AGT-024), (1:400), followed by goat-anti-rabbit-AlexaFluor-488. GLUT4 staining (green) appears in some glomeruli (“G” and horizontal arrows), but not in others (vertical arrow). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KPSTELEYLGPDE, corresponding to amino acid residues 495-507 of human Glucose Transporter 4 (Accession P14672).
Accession (Uniprot) Number P14672
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 3 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
