Overview
Type: Synthetic peptide
Form: Lyophilized powder
GLUT5/SLC2A5 Blocking Peptide (#BLP-GT025) is the original antigen used for immunization during Anti-GLUT5 (SLC2A5) Antibody (#AGT-025) generation. The blocking peptide binds and ‘blocks’ Anti-GLUT5/SLC2A5 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1,2. Anti-GLUT5 (SLC2A5) Antibody (#AGT-025), (1:400).
3,4. Anti-GLUT5 (SLC2A5) Antibody, preincubated with GLUT5/SLC2A5 Blocking Peptide (#BLP-GT025).
Western blot analysis of rat small intestine lysate:1. Anti-GLUT5 (SLC2A5) Antibody (#AGT-025), (1:200).
2. Anti-GLUT5 (SLC2A5) Antibody, preincubated with GLUT5/SLC2A5 Blocking Peptide (#BLP-GT025).
Western blot analysis of human MCF-7 breast adenocarcinoma cell lysates (lanes 1 and 3) and human Caco-2 colon adenocarcinoma cell lysates (lanes 2 and 4):1,2. Anti-GLUT5 (SLC2A5) Antibody (#AGT-025), (1:200).
3,4. Anti-GLUT5 (SLC2A5) Antibody, preincubated with GLUT5/SLC2A5 Blocking Peptide (#BLP-GT025).
Expression of Glucose Transporter 5 in mouse hippocampus and cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GLUT5 (SLC2A5) Antibody (#AGT-025), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. GLUT5 staining (green) in hippocampal CA3 region, is detected in cells of the pyramidal layer (P) and in their apical dendrites (arrows). B. GLUT5 staining (green) in cerebellum appears in cells of the Purkinje layer (P) and in their apical dendrites (arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KNKVSDVYPEKEEK, corresponding to amino acid residues 477 - 490 of rat Glucose Transporter 5 (Accession P43427).
Accession (Uniprot) Number P43427
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
