Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)KNKVSDVYPEKEEK, corresponding to amino acid residues 477 - 490 of rat Glucose Transporter 5 (Accession P43427).
Accession (Uniprot) Number P43427
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1,2. Anti-GLUT5 (SLC2A5) Antibody (#AGT-025), (1:400).
3,4. Anti-GLUT5 (SLC2A5) Antibody, preincubated with GLUT5/SLC2A5 Blocking Peptide (#BLP-GT025).
Western blot analysis of rat small intestine lysate:1. Anti-GLUT5 (SLC2A5) Antibody (#AGT-025), (1:200).
2. Anti-GLUT5 (SLC2A5) Antibody, preincubated with GLUT5/SLC2A5 Blocking Peptide (#BLP-GT025).
Western blot analysis of human MCF-7 breast adenocarcinoma cell lysates (lanes 1 and 3) and human Caco-2 colon adenocarcinoma cell lysates (lanes 2 and 4):1,2. Anti-GLUT5 (SLC2A5) Antibody (#AGT-025), (1:200).
3,4. Anti-GLUT5 (SLC2A5) Antibody, preincubated with GLUT5/SLC2A5 Blocking Peptide (#BLP-GT025).- Mouse brain sections (1:200).
Last update: 02/01/2024
