Overview
Type: Synthetic peptide
Form: Lyophilized Powder
GLUT8 (extracellular) Blocking Peptide (#BLP-GT028) is the original antigen used for immunization during Anti-GLUT8 (extracellular) Antibody (#AGT-028) generation. The blocking peptide binds and ‘blocks’ Anti-GLUT8 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 4), mouse liver membranes (lanes 2 and 5) and mouse testis membranes (lanes 3 and 6):1-3. Anti-GLUT8 (extracellular) Antibody (#AGT-028), (1:200).
4-6. Anti-GLUT8 (extracellular) Antibody, preincubated with GLUT8 (extracellular) Blocking Peptide (BLP-GT028).
Expression of GLUT8 in mouse small intestine.Immunohistochemical staining of formalin-fixed paraffin-embedded (FFPE) mouse small intestine sections, with Anti-GLUT8 (extracellular) Antibody (#AGT-028), (1:100), followed by goat anti-rabbit-AlexaFluor-647. A. GLUT8 immunoreactivity (red) appears in goblet cells of the small intestine (arrows). B. Pre-incubation of the antibody with GLUT8 Blocking Peptide (#BLP-GT028), suppressed staining. Cell nuclei are stained with DAPI (blue). Heat-induced epitope retrieval was done with citrate buffer, pH6.
Properties
Sequence
- Peptide (C)KLTQSGPSNSSHVG, corresponding to amino acid residues 342 - 355 of rat GLUT8 (Accession Q9JJZ1).
Accession (Uniprot) Number Q9JJZ1
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
