Cat #: AK-440_KIT
Size: 16 Vials
Form: Lyophilized
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Anti-EAAT1 (GLAST) (extracellular) Antibody Cat #: AGC-021
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)KQFKTSYEKRSFK, corresponding to amino acids 188-200 of rat EAAT1 (Accession P24942). 2nd extracellular loop.
Homology
Mouse - identical; human 12/13 amino acid residues identical.
EAAT1/GLAST (extracellular) Blocking Peptide Cat #: BLP-GC021
Size:
40 µg
Anti-EAAT2 (GLT-1) (extracellular) Antibody Cat #: AGC-022
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)KQLGPGKKNDEVS, corresponding to amino acid residues 151-163 of rat EAAT2 (Accession P31596). 2nd extracellular loop.
Homology
Mouse, human - identical.
EAAT2/GLT-1 (extracellular) Blocking Peptide Cat #: BLP-GC022
Size:
40 µg
Anti-EAAT3 (EAAC1) Antibody Cat #: AGC-023
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)EPTILDNEDSDTKK, corresponding to amino acid residues 487-500 of rat EAAT3 (Accession P51907). Intracellular, C-terminus.
Homology
Mouse, human - 13/14 amino acid residues identical.
EAAT3/EAAC1 Blocking Peptide Cat #: BLP-GC023
Size:
40 µg
Anti-EAAT4 (extracellular) Antibody Cat #: AGC-024
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
WB
Reactivity:
M, R
Immunogen
Peptide (C)TRTIVRTDNGSE, corresponding to amino acid residues 205-216 of rat EAAT4 (Accession O35921). 2nd extracellular loop.
Homology
Mouse - identical; human - 10/12 amino acid residues identical.
EAAT4 (extracellular) Blocking Peptide Cat #: BLP-GC024
Size:
40 µg
Anti-SLC1A7 (extracellular) Antibody Cat #: AGC-025
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
WB
Reactivity:
M, R
Immunogen
Peptide (C)KETTEQSGKPVMS, corresponding to amino acid residues 122-134 of mouse EAAT5 (Accession Q8JZR4). 2nd extracellular loop.
Homology
Rat – identical; human – 12/13 amino acid residues identical.
SLC1A7 (extracellular) Blocking Peptide Cat #: BLP-GC025
Size:
40 µg
Anti-VGLUT1 Antibody Cat #: AGC-035
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)EFRQEEFRKLAGR, corresponding to amino acid residues 2-14 of rat VGLUT1 (Accession Q62634). Cytoplasmic, N-terminus.
Homology
Mouse, human – identical.
VGLUT1 Blocking Peptide Cat #: BLP-GC035
Size:
40 µg
Anti-VGLUT2 Antibody Cat #: AGC-036
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)EDGKPLEVPEKK, corresponding to amino acid residues 45-56 of rat VGLUT2 (Accession Q9JI12). Cytoplasmic, N-terminus.
Homology
Mouse - identical; human - 11/12 amino acid residues identical.
VGLUT2 Blocking Peptide Cat #: BLP-GC036
Size:
40 µg
Anti-VGLUT3 Antibody Cat #: AGC-037
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
M, R
Immunogen
Peptide (C)ELNHEAFVSPRKK, corresponding to amino acid residues 533-545 of rat VGLUT3 (Accession Q7TSF2). Cytoplasmic, C-terminus.
Homology
Mouse - 12/13 amino acid residues identical; human - 10/13 amino acid residues identical.
VGLUT3 Blocking Peptide Cat #: BLP-GC037
Size:
40 µg
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
For research purposes only, not for human use
