Overview
Type: Synthetic peptide
Form: Lyophilized powder
Glycine Receptor α1 Blocking Peptide (#BLP-GR001) is the original antigen used for immunization during Anti-Glycine Receptor α1 Antibody (#AGR-001) generation. The blocking peptide binds and ‘blocks’ Anti-Glycine Receptor α1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat DRG (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat brain (lanes 3 and 6) lysates:1-3. Anti-Glycine Receptor α1 Antibody (#AGR-001), (1:400).
4-6. Anti-Glycine Receptor α1 Antibody, preincubated with Glycine Receptor α1 Blocking Peptide (#BLP-GR001).
Expression of GlyR α1 in rat cerebellumImmunohistochemical staining of rat cerebellum using Anti-Glycine receptor α1 Antibody (#AGR-001), (1:100). A. GlyRα1 staining (red) is revealed in the molecular layer (MOL), Bergmann glia (horizontal arrow) and in Purkinje cells (a vertical arrow). B. Nuclear staining using DAPI as the counterstain (blue). C. Merged images of A and B.
Properties
Sequence
- (C)RHHKSPMLNLFQD, corresponding to amino acid residues 350-362 of rat GlyRα1 (Accession P07727).
Accession (Uniprot) Number P07727
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
