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Cat #: AK-230_KIT
Size: 8 Vials
Form: Lyophilized
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Anti-Glycine Receptor α1 Antibody Cat #: AGR-001
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IF, IH, WB
Reactivity:
M, R
Immunogen
Peptide (C)RHHKSPMLNLFQD, corresponding to amino acid residues 350-362 of rat GlyRα1 (Accession P07727). 2nd intracellular loop.
Homology
Mouse - identical; human - 12/13 amino acid residues identical.
Glycine Receptor α1 Blocking Peptide Cat #: BLP-GR001
Size:
40 µg
Anti-Glycine Receptor α3 Antibody Cat #: AGR-003
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IH, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)KNKTEAFALEKFYR, corresponding to amino acid residues 355-368 of rat GlyRα3 (Accession P24524). 2nd intracellular loop.
Homology
Mouse, human - identical.
Glycine Receptor α3 Blocking Peptide Cat #: BLP-GR003
Size:
40 µg
Anti-Glycine Receptor α4 (extracellular) Antibody Cat #: AGR-015
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)KEDVKSGLKGSQPMS, corresponding to amino acid residues 28-42 of mouse GlyRα4 (Accession Q61603). Extracellular, N-terminus.
Homology
Rat - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical.
Glycine Receptor α4 (extracellular) Blocking Peptide Cat #: BLP-GR015
Size:
40 µg
Anti-Glycine Receptor β Antibody Cat #: AGR-014
Size:
50 µl
Host: Rabbit
Type: Polyclonal
Application:
IH, WB
Reactivity:
H, M, R
Immunogen
Peptide HISTLQVGETRC, corresponding to amino acid residues 394-405 of rat GlyRβ (Accession P20781). 2nd intracellular loop.
Homology
Mouse, human – identical.
Glycine Receptor β Blocking Peptide Cat #: BLP-GR014
Size:
40 µg
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
For research purposes only, not for human use
