Overview
Type: Synthetic peptide
Form: Lyophilized powder
GlyT2/SLC6A5 (extracellular) Blocking Peptide (#BLP-GT012) is the original antigen used for immunization during Anti-GlyT2 (SLC6A5) (extracellular) Antibody (#AGT-012) generation. The blocking peptide binds and ‘blocks’ Anti-GlyT2/SLC6A5 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain (lanes 1 and 4), rat brain (lanes 2 and 5) and rat cerebellum (lanes 3 and 6) lysates:1-3. Anti-GlyT2 (SLC6A5) (extracellular) Antibody (#AGT-012), (1:200).
4-6. Anti-GlyT2 (SLC6A5) (extracellular) Antibody, preincubated with GlyT2/SLC6A5 (extracellular) Blocking Peptide (#BLP-GT012).
Expression of glycine transporter 2 in mouse brain stemImmunohistochemical staining of immersion-fixed, free floating mouse brain frozen sections using Anti-GlyT2 (SLC6A5) (extracellular) Antibody (#AGT-012), (1:200). A. GlyT2 (red) is expressed in neurons (arrow). B. Dapi staining of cell nuclei (blue) is used as a general cellular marker.
Properties
Sequence
- CVIGDHPKIQIKNS, corresponding to amino acid residues 333-346 of rat GlyT2 (Accession P58295).
Accession (Uniprot) Number P58295
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
