Cat #: BLP-GT012
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- CVIGDHPKIQIKNS, corresponding to amino acid residues 333-346 of rat GlyT2 (Accession P58295).
Accession (Uniprot) Number P58295
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of mouse brain (lanes 1 and 4), rat brain (lanes 2 and 5) and rat cerebellum (lanes 3 and 6) lysates:1-3. Anti-GlyT2 (SLC6A5) (extracellular) Antibody (#AGT-012), (1:200).
4-6. Anti-GlyT2 (SLC6A5) (extracellular) Antibody, preincubated with GlyT2/SLC6A5 (extracellular) Blocking Peptide (#BLP-GT012). - Mouse brain stem (1:200).
Last update: 02/01/2024