Overview
Type: Synthetic peptide
Form: Lyophilized powder
GnRH Receptor (extracellular) Blocking Peptide (#BLP-GR030) is the original antigen used for immunization during Anti-GnRH Receptor (extracellular) Antibody (#AGR-030) generation. The blocking peptide binds and ‘blocks’ Anti-GnRH Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat ovary (lanes 1 and 3) and pituitary (lanes 2 and 4) lysates:1,2. Anti-GnRH Receptor (extracellular) Antibody (#AGR-030), (1:200).
3,4. Anti-GnRH Receptor (extracellular) Antibody, preincubated with GnRH Receptor (extracellular) Blocking Peptide (#BLP-GR030).
Expression of GnRHR in rat ovaryImmunohistochemical staining of rat ovary paraffin-embedded sections using Anti-GnRH Receptor (extracellular) Antibody (#AGR-030). GnRHR labeling appears in the stroma surrounding the follicles. The stroma is a richly cellular connective tissue with scattered smooth muscle fibers. The theca layer is stained as well. Hematoxilin is used as the counterstain.
Properties
Sequence
- (C)NNASLEQDQNH, corresponding to amino acid residues 3-13 of rat GnRHR (Accession P30969).
Accession (Uniprot) Number P30969
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
