Overview
Type: Synthetic peptide
Form: Lyophilized powder
GP130 (extracellular) Blocking Peptide (#BLP-LR023) is the original antigen used for immunization during Anti-GP130 (extracellular) Antibody (#ALR-023) generation. The blocking peptide binds and ‘blocks’ Anti-GP130 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membrane (lanes 1 and 5), rat brain lysate (lanes 2 and 6), rat brain membrane (lanes 3 and 7) and rat uterus lysate (lanes 4 and 8):1-3. Anti-GP130 (extracellular) Antibody (#ALR-023), (1:400).
4. Anti-GP130 (extracellular) Antibody (1:200).
5-8. Anti-GP130 (extracellular) Antibody, preincubated with GP130 (extracellular) Blocking Peptide (#BLP-LR023).
Expression of GP130/CD130 in rat subfornical organ (SFO).Immunohistochemical staining of perfusion-fixed frozen rat brain sections Anti-GP130 (extracellular) Antibody (#ALR-023), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. A. GP130/CD130 immunoreactivity (green) appears in the SFO (arrows). B. Pre-incubation of the antibody with GP130 (extracellular) Blocking Peptide (BLP-LR023), suppressed staining. Cell nuclei are stained with DAPI (blue). FX = fornix; V = cerebral ventricle.
Properties
Sequence
- (C)DTLYMVHMAAYTEEG, corresponding to amino acid residues 584-598 of rat GP130 (Accession P40190).
Accession (Uniprot) Number P40190
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
