Overview
Type: Synthetic peptide
Form: Lyophilized powder
GPER1/GPR30 (extracellular) Blocking Peptide (#BLP-ER049) is the original antigen used for immunization during Anti-GPER1/GPR30 (extracellular) Antibody (#AER-049) generation. The blocking peptide binds and ‘blocks’ Anti-GPER1/GPR30 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-GPER1/GPR30 (extracellular) Antibody (#AER-049), (1:200).
3,4. Anti-GPER1/GPR30 (extracellular) Antibody, preincubated with GPER1/GPR30 (extracellular) Blocking Peptide (#BLP-ER049).
Western blot analysis of human MCF-7 breast adenocarcinoma (lanes 1 and 4), human THP-1 monocytic leukemia (lanes 2 and 5) and human Jurkat T-cell leukemia (lanes 3 and 6) cell line lysates:1-3. Anti-GPER1/GPR30 (extracellular) Antibody (#AER-049), (1:200).
4-6. Anti-GPER1/GPR30 (extracellular) Antibody, preincubated with GPER1/GPR30 (extracellular) Blocking Peptide (#BLP-ER049).
Properties
Sequence
- CKQSFRHAYPLTGH, corresponding to amino acid residues 294 - 307 of mouse GPER1 (Accession Q8BMP4).
Accession (Uniprot) Number Q8BMP4
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
