Overview
Type: Synthetic peptide
Form: Lyophilized Powder
GPR108 (extracellular) Blocking Peptide (#BLP-GR088) is the original antigen used for immunization during Anti-GPR108 (extracellular) Antibody (#AGR-088) generation. The blocking peptide binds and ‘blocks’ Anti-GPR108 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Anti-GPR108 (extracellular) Antibody (#AGR-088), (1:200).
3-4. Anti-GPR108 (extracellular) Antibody, preincubated with GPR108 (extracellular) Blocking Peptide (BLP-GR088).
Western blot analysis of mouse TK-1 T-cell lymphoma cell line lysate (lanes 1 and 4), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 5) and mouse BV-2 microglia cell line lysate (lanes 3 and 6):1-3. Anti-GPR108 (extracellular) Antibody (#AGR-088), (1:200).
4-6. Anti-GPR108 (extracellular) Antibody, preincubated with GPR108 (extracellular) Blocking Peptide (BLP-GR088).
Expression of GPR108 in mouse red nucleus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR108 (extracellular) Antibody (#AGR-088), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. GPR108 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with GPR108 (extracellular) Blocking Peptide (BLP-GR088), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)KDLQVQVRKYGEQK, corresponding to amino acid residues 124 - 137 of mouse GPR108 (Accession Q91WD0).
Accession (Uniprot) Number Q91WD0
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
