Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized Powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- Peptide (C)DNYVHNWDWRFGG, corresponding to amino acid residues 82 - 94 of mouse HCAR2 (Accession Q9EP66).
Accession (Uniprot) Number Q9EP66
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat spleen lysates (lanes 1 and 3) and rat colon membranes (lanes 2 and 4):1-2. Anti-GPR109A/HCAR2 (extracellular) Antibody (#AHR-012), (1:200).
3-4. Anti-GPR109A/HCAR2 (extracellular) Antibody, preincubated with GPR109A/HCAR2 (extracellular) Blocking Peptide (BLP-HR012).
Western blot analysis of mouse colon lysates (lanes 1 and 3) and mouse lung lysates (lanes 2 and 4):1-2. Anti-GPR109A/HCAR2 (extracellular) Antibody (#AHR-012), (1:200).
3-4. Anti-GPR109A/HCAR2 (extracellular) Antibody, preincubated with GPR109A/HCAR2 (extracellular) Blocking Peptide (BLP-HR012).
Western blot analysis of human THP-1 monocytic leukemia cell line lysates (lanes 1 and 3) and human HL-60 promyelocytic leukemia cell line lysates (lanes 2 and 4):1-2. Anti-GPR109A/HCAR2 (extracellular) Antibody (#AHR-012), (1:200).
3-4. Anti-GPR109A/HCAR2 (extracellular) Antibody, preincubated with GPR109A/HCAR2 (extracellular) Blocking Peptide (BLP-HR012).
Last update: 02/01/2024
