Overview
Type: Synthetic peptide
Form: Lyophilized powder
GPR120/FFAR4 (extracellular) Blocking Peptide (#BLP-FR014) is the original antigen used for immunization during Anti-GPR120/FFAR4 (extracellular) Antibody (#AFR-014) generation. The blocking peptide binds and ‘blocks’ Anti-GPR120/FFAR4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat lung (lanes 1 and 3), rat tongue (lanes 2 and 4), human U-87 astrocytoma cells (lanes 5 and 7) and mouse MS1 endothelial cells (lanes 6 and 8) lysates:1,2,5,6. Anti-GPR120/FFAR4 (extracellular) Antibody (#AFR-014), (1:200).
3,4,7,8. Anti-GPR120/FFAR4 (extracellular) Antibody, preincubated with GPR120/FFAR4 (extracellular) Blocking Peptide (#BLP-FR014).
Western blot analysis of mouse colon (lanes 9 and 11) and human colorectal adenocarcinoma (Colo 205) cell line (lanes 10 and 12) lysates:9,10. Anti-GPR120/FFAR4 (extracellular) Antibody (#AFR-014), (1:200).
11,12. Anti-GPR120/FFAR4 (extracellular) Antibody, preincubated with GPR120/FFAR4 (extracellular) Blocking Peptide (#BLP-FR014).
Expression of FFAR4 in rat hypothalamus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR120/FFAR4 (extracellular) Antibody (#AFR-014), (1:1200), followed by biotinylated donkey anti-rabbit and streptavidin-Cy3. A. FFAR4 immunoreactivity (red) appears in axons beaded with varicosities (arrows). . B. Pre-incubation of the antibody with GPR120/FFAR4 (extracellular) Blocking Peptide (BLP-FR014), suppressed staining. Cell nuclei are stained with DAPI (blue). VMH = ventromedial hypothalamus, 3V = 3rd ventricle
Properties
Sequence
- (C)RTHFPFFSDVKGDHR, corresponding to amino acid residues 22-36 of mouse FFAR4 (Accession Q7TMA4).
Accession (Uniprot) Number Q7TMA4
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
