Overview
Type: Synthetic peptide
Form: Lyophilized powder
GPR126/ADGRG6 (extracellular) Blocking Peptide (#BLP-GR056) is the original antigen used for immunization during Anti-GPR126/ADGRG6 (extracellular) Antibody (#AGR-056) generation. The blocking peptide binds and ‘blocks’ Anti-GPR126/ADGRG6 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse lung lysate (lanes 1 and 3) and mouse heart lysate (lanes 2 and 4):1, 2. Anti-GPR126/ADGRG6 (extracellular) Antibody (#AGR-056), (1:200).
3, 4. Anti-GPR126/ADGRG6 (extracellular) Antibody, preincubated with GPR126/ADGRG6 (extracellular) Blocking Peptide (#BLP-GR056) (#BLP-GR056).
Western blot analysis of rat brain membranes:1. Anti-GPR126/ADGRG6 (extracellular) Antibody (#AGR-056), (1:200).
2. Anti-GPR126/ADGRG6 (extracellular) Antibody, preincubated with GPR126/ADGRG6 (extracellular) Blocking Peptide (#BLP-GR056) (#BLP-GR056).
Western blot analysis of human LNCaP prostate carcinoma cell line lysate (lanes 1 and 5), human MCF-7 breast adenocarcinoma cell line lysate (lanes 2 and 6), human Malme-3M melanoma cell line lysate (lanes 3 and 7) and human K562 erythroleukemia cell line lysate (lanes 4 and 8):1-4. Anti-GPR126/ADGRG6 (extracellular) Antibody (#AGR-056), (1:200).
5-8. Anti-GPR126/ADGRG6 (extracellular) Antibody, preincubated with blocking peptide.
Properties
Sequence
- (C)KDPVQIKIKHTRTQE, corresponding to amino acid residues 753 - 767 of mouse GPR126 (Accession Q86SQ4).
Accession (Uniprot) Number Q86SQ4
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
