Overview
Type: Synthetic peptide
Form: Lyophilized Powder
GPR158 Blocking Peptide (#BLP-GR078) is the original antigen used for immunization during Anti-GPR158 Antibody (#AGR-078) generation. The blocking peptide binds and ‘blocks’ Anti-GPR158 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1-2. Anti-GPR158 Antibody (#AGR-078), (1:200).
3-4. Anti-GPR158 Antibody, preincubated with GPR158 Blocking Peptide (BLP-GR078).
Western blot analysis of human PC-3 prostate adenocarcinoma cell line lysate (lanes 1 and 4), human U-87 MG glioblastoma cell line lysate (lanes 2 and 5) and human OVCAR-3 ovarian carcinoma cell line lysate (lanes 3 and 6):1-3. Anti-GPR158 Antibody (#AGR-078), (1:200).
4-6. Anti-GPR158 Antibody, preincubated with GPR158 Blocking Peptide (BLP-GR078).
Expression of GPR158 in rat cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-GPR158 Antibody (#AGR-078), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. GPR158 immunoreactivity (green) appears in the molecular layer and around purkinje cell soma (arrows). B. Pre-incubation of the antibody with GPR158 Blocking Peptide (BLP-GR078), suppressed staining. Cell nuclei are stained with DAPI (blue). M = molecular layer, G = granule layer.
Properties
Sequence
- Peptide (C)EPRKPQKSGIMKQQR, corresponding to amino acid residues 960 - 974 of mouse GPR158 (Accession Q8C419).
Accession (Uniprot) Number Q8C419
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
