Overview
Type: Synthetic peptide
Form: Lyophilized powder
GPR17 Blocking Peptide (#BLP-GR048) is the original antigen used for immunization during Anti-GPR17 Antibody (#AGR-048) generation. The blocking peptide binds and ‘blocks’ Anti-GPR17 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1,2. Anti-GPR17 Antibody (#AGR-048), (1:200).
3,4. Anti-GPR17 Antibody, preincubated with GPR17 Blocking Peptide (#BLP-GR048).
Expression of GPR17 in rat parietal cortexImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR17 Antibody (#AGR-048), (1:300), followed by donkey-anti-rabbit-biotin and streptavidin-Cy3. GPR17 immunoreactivity (red) appears in cortical cells (arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RQGPRIEKHLKNK, corresponding to amino acid residues 220-232 of rat GPR17 (Accession Q09QM4).
Accession (Uniprot) Number Q09QM4
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
