Overview
Type: Synthetic peptide
Form: Lyophilized Powder
GPR174 (extracellular) Blocking Peptide (#BLP-GR060) is the original antigen used for immunization during Anti-GPR174 (extracellular) Antibody (#AGR-060) generation. The blocking peptide binds and ‘blocks’ Anti-GPR174 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain lysate (lanes 2 and 6), rat spleen lysate (lanes 3 and 7) and mouse spleen membranes (lanes 4 and 8):1-4. Anti-GPR174 (extracellular) Antibody (#AGR-060), (1:400).
5-8. Anti-GPR174 (extracellular) Antibody, preincubated with GPR174 (extracellular) Blocking Peptide (#BLP-GR060).
Western blot analysis of human MOLT-4 T-cell leukemia cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4):1, 2. Anti-GPR174 (extracellular) Antibody (#AGR-060), (1:400).
3, 4. Anti-GPR174 (extracellular) Antibody, preincubated with GPR174 (extracellular) Blocking Peptide (#BLP-GR060).
Properties
Sequence
- Peptide (C)DTSGNRTK(S)FVDLPTR, corresponding to amino acid residues 160 - 175 of human GPR174 (Accession Q9BXC1).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 2 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
