Cat #: BLP-GR068
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized Powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- Peptide (C)HNQLDLSNGSHPEEYK, corresponding to amino acid residues 7 - 22 of mouse GPR18 (Accession Q8K1Z6).
Accession (Uniprot) Number Q8K1Z6
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Anti-GPR18 (extracellular) Antibody (#AGR-068), (1:200).
3-4. Anti-GPR18 (extracellular) Antibody, preincubated with GPR18 (extracellular) Blocking Peptide (BLP-GR068). - Western blot analysis of mouse EL4 T-cell lymphoma cell line lysate (lanes 1 and 4), mouse WEHI-231 B-cell lymphoma cell line lysate (lanes 2 and 5) and mouse BV-2 microglia cell line lysate (lanes 3 and 6):1-3. Anti-GPR18 (extracellular) Antibody (#AGR-068), (1:200).
4-6. Anti-GPR18 (extracellular) Antibody, preincubated with GPR18 (extracellular) Blocking Peptide (BLP-GR068).
Last update: 25/07/2024