Overview
Type: Synthetic peptide
Form: Lyophilized Powder
GPR183 (extracellular) Blocking Peptide (#BLP-GR063) is the original antigen used for immunization during Anti-GPR183 (extracellular) Antibody (#AGR-063) generation. The blocking peptide binds and ‘blocks’ Anti-GPR183 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain lysates (lanes 2 and 5) and rat lung membranes (lanes 3 and 6):1-3. Anti-GPR183 (extracellular) Antibody (#AGR-063), (1:200).
4-6. Anti-GPR183 Antibody, preincubated with GPR183 (extracellular) Blocking Peptide (BLP-GR063).
Western blot analysis of rat spleen membranes (lanes 1 and 3) and mouse spleen membranes (lanes 2 and 4):1-2. Anti-GPR183 (extracellular) Antibody (#AGR-063), (1:200).
3-4. Anti-GPR183 Antibody, preincubated with GPR183 (extracellular) Blocking Peptide (BLP-GR063).
Western blot analysis of human Daudi B-lymphoblast cell line lysates (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysates (lanes 2 and 4):1-2. Anti-GPR183 (extracellular) Antibody (#AGR-063), (1:200).
3-4. Anti-GPR183 Antibody, preincubated with GPR183 (extracellular) Blocking Peptide (BLP-GR063).
Expression of GPR183 in in rat parietal cortex.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR183 (extracellular) Antibody (#AGR-063), (1:1200), followed by donkey anti-rabbit-biotin and streptavidin-Cy3. A. GPR183 immunoreactivity (red) appears in soma and apical dendrite profiles (arrows). B. Pre-incubation of the antibody with GPR183 (extracellular) Blocking Peptide (BLP-GR063), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)GND(S)DLYAHHSTAR, corresponding to amino acid residues 18 - 31 of human GPR183 (Accession P32249).
Accession (Uniprot) Number P32249
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
