Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized Powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- Peptide (C)GND(S)DLYAHHSTAR, corresponding to amino acid residues 18 - 31 of human GPR183 (Accession P32249).
Accession (Uniprot) Number P32249
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain lysates (lanes 2 and 5) and rat lung membranes (lanes 3 and 6):1-3. Anti-GPR183 (extracellular) Antibody (#AGR-063), (1:200).
4-6. Anti-GPR183 Antibody, preincubated with GPR183 (extracellular) Blocking Peptide (BLP-GR063).
Western blot analysis of rat spleen membranes (lanes 1 and 3) and mouse spleen membranes (lanes 2 and 4):1-2. Anti-GPR183 (extracellular) Antibody (#AGR-063), (1:200).
3-4. Anti-GPR183 Antibody, preincubated with GPR183 (extracellular) Blocking Peptide (BLP-GR063).
Western blot analysis of human Daudi B-lymphoblast cell line lysates (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysates (lanes 2 and 4):1-2. Anti-GPR183 (extracellular) Antibody (#AGR-063), (1:200).
3-4. Anti-GPR183 Antibody, preincubated with GPR183 (extracellular) Blocking Peptide (BLP-GR063).
Last update: 02/01/2024
