Overview
Type: Synthetic peptide
Form: Lyophilized powder
GPR34 (extracellular) Blocking Peptide (#BLP-GR055) is the original antigen used for immunization during Anti-GPR34 (extracellular) Antibody (#AGR-055) generation. The blocking peptide binds and ‘blocks’ Anti-GPR34 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1,2. Anti-GPR34 (extracellular) Antibody (#AGR-055), (1:200).
3,4. Anti-GPR34 (extracellular) Antibody, preincubated with GPR34 (extracellular) Blocking Peptide (#BLP-GR055).
Western blot analysis of mouse BV-2 microglia cell line lysate (lanes 1 and 3) and human THP-1 monocytic leukemia cell line lysate (lanes 2 and 4):1,2. Anti-GPR34 (extracellular) Antibody (#AGR-055), (1:200).
3,4. Anti-GPR34 (extracellular) Antibody, preincubated with GPR34 (extracellular) Blocking Peptide (#BLP-GR055).
Properties
Sequence
- CFHYRDRHNAKGE, corresponding to amino acid residues 197 - 209 of mouse GPR34 (Accession Q9R1K6).
Accession (Uniprot) Number Q9R1K6
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
