Overview
Type: Synthetic peptide
Form: Lyophilized Powder
GPR35 (extracellular) Blocking Peptide (#BLP-GR051) is the original antigen used for immunization during Anti-GPR35 (extracellular) Antibody (#AGR-051) generation. The blocking peptide binds and ‘blocks’ Anti-GPR35 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1-2. Anti-GPR35 (extracellular) Antibody (#AGR-051), (1:200). 3-4. Anti-GPR35 (extracellular) Antibody, preincubated with GPR35 (extracellular) Blocking Peptide (BLP-GR051).
Western blot analysis of rat dorsal root ganglion lysate:1. Anti-GPR35 (extracellular) Antibody (#AGR-051), (1:200). 2. Anti-GPR35 (extracellular) Antibody, preincubated with GPR35 (extracellular) Blocking Peptide (BLP-GR051).
Western blot analysis of rat small intestine lysate:1. Anti-GPR35 (extracellular) Antibody (#AGR-051), (1:200). 2. Anti-GPR35 (extracellular) Antibody, preincubated with GPR35 (extracellular) Blocking Peptide (BLP-GR051).
Expression of GPR35 in rat dorsal root ganglion.Immunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglion (DRG) sections with Anti-GPR35 (extracellular) Antibody (#AGR-051), followed by goat anti-rabbit-AlexaFluor-488. A. GPR35 immunoreactivity (green) appeared in neuron profiles (arrows). B. Pre-incubation of the antibody with GPR35 (extracellular) Blocking Peptide (#BLP-GR051), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)EGGF(S)FSSQTRRNFS, corresponding to amino acid residues 156 - 170 of mouse GPR35 (Accession Q9ES90).
Accession (Uniprot) Number Q9ES90
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
