Overview
Type: Synthetic peptide
Form: Lyophilized Powder
GPR37L1 (extracellular) Blocking Peptide (#BLP-GR050) is the original antigen used for immunization during Anti-GPR37L1 (extracellular) Antibody (#AGR-050) generation. The blocking peptide binds and ‘blocks’ Anti-GPR37L1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1-2. Anti-GPR37L1 (extracellular) Antibody (#AGR-050), (1:200). 3-4. Anti-GPR37L1 (extracellular) Antibody, preincubated with GPR37L1 (extracellular) Blocking Peptide (BLP-GR050).
Western blot analysis of human U-87 MG glioblastoma cell line lysate:1. Anti-GPR37L1 (extracellular) Antibody (#AGR-050), (1:200). 2. Anti-GPR37L1 (extracellular) Antibody, preincubated with GPR37L1 (extracellular) Blocking Peptide (BLP-GR050).
Expression of GPR37L1 in rat cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR37L1 (extracellular) Antibody (#AGR-050), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. GPR37L1 immunoreactivity (green) appeared around Purkinje cell soma (arrows) and in the molecular layer (M). B. Pre-incubation of the antibody with GPR37L1 (extracellular) Blocking Peptide (BLP-GR050), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)EYPKPIHPAGLQPTK, corresponding to amino acid residues 66 - 80 of rat GPR37L1 (Accession Q9QYC5).
Accession (Uniprot) Number Q9QYC5
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
