Overview
Type: Synthetic peptide
Form: Lyophilized powder
GPR4 (extracellular) Blocking Peptide (#BLP-GR041) is the original antigen used for immunization during Anti-GPR4 (extracellular) Antibody (#AGR-041) generation. The blocking peptide binds and ‘blocks’ Anti-GPR4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat dorsal root ganglion lysate:1. Anti-GPR4 (extracellular) Antibody (#AGR-041), (1:200).
2. Anti-GPR4 (extracellular) Antibody, preincubated with GPR4 (extracellular) Blocking Peptide (#BLP-GR041).
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) kidney membranes:1,2. Anti-GPR4 (extracellular) Antibody (#AGR-041), (1:200).
3,4. Anti-GPR4 (extracellular) Antibody, preincubated with GPR4 (extracellular) Blocking Peptide (#BLP-GR041).
Expression of GPR4 in rat dorsal raphe nucleus (DRN).Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR4 (extracellular) Antibody (#AGR-041), (1:400), followed by goat anti-rabbit-Alexa-488. A. GPR4 immunoreactivity (green) appears in cells of both medial and lateral DRN (arrows). B. Pre-incubation of the antibody with GPR4 (extracellular) Blocking Peptide (#BLP-GR041), suppressed staining. Cell nuclei are stained with DAPI (blue). LDRN = lateral DRN, MDRN = medial DRN.
Properties
Sequence
- (C)RDRYNHTF(S)FEKFPME, corresponding to amino acid residues 162-177 of rat GPR4 (Accession Q4KLH9).
Accession (Uniprot) Number Q4KLH9
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
