Overview
Cat #: BLP-FR011
Type: Synthetic peptide
Form: Lyophilized powder
GPR40/FFAR1 (extracellular) Blocking Peptide (#BLP-FR011) is the original antigen used for immunization during Anti-GPR40/FFAR1 (extracellular) Antibody (#AFR-011) generation. The blocking peptide binds and ‘blocks’ Anti-GPR40/FFAR1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat pancreas lysate:1. Anti-GPR40/FFAR1 (extracellular) Antibody (#AFR-011), (1:200).
2. Anti-GPR40/FFAR1 (extracellular) Antibody, preincubated with GPR40/FFAR1 (extracellular) Blocking Peptide (#BLP-FR011). - Expression of GPR40/FFAR1 in rat brainImmunohistochemical staining of rat brain sections using Anti-GPR40/FFAR1 (extracellular) Antibody (#AFR-011). A. GPR40 immunoreactivity (green) in the cerebellum appears in the molecular layer (MOL) and in Purkinje cells (arrows). Nuclei are demonstrated using DAPI as the counterstain (blue).
Properties
Sequence
- (C)NVASFINPDLEGSWRK corresponding to amino acid residues 244-259 of rat FFAR1 (Accession Q8K3T4).
Accession (Uniprot) Number Q8K3T4
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.