Overview
Type: Synthetic peptide
Form: Lyophilized powder
GPR52 Blocking Peptide (#BLP-GR058) is the original antigen used for immunization during Anti-GPR52 Antibody (#AGR-058) generation. The blocking peptide binds and ‘blocks’ Anti-GPR52 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysates (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):1, 2. Anti-GPR52 Antibody (#AGR-058), (1:200).
3, 4. Anti-GPR52 Antibody, preincubated with GPR52 Blocking Peptide (#BLP-GR058) (#BLP-GR058).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1. Anti-GPR52 Antibody (#AGR-058), (1:200).
2. Anti-GPR52 Antibody, preincubated with GPR52 Blocking Peptide (#BLP-GR058) (#BLP-GR058).
Expression of GPR52 in rat substantia nigraImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR52 Antibody (#AGR-058), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. GPR52 immunoreactivity (green) appears in neuronal profiles (arrows). B. Pre-incubation of the antibody with GPR52 Blocking Peptide (#BLP-GR058), suppresses staining. Cell nuclei are stained with DAPI (blue). SNR = substantia nigra pars reticulata.
Properties
Sequence
- (C)DRRARFPSHEVEASR, corresponding to amino acid residues 239 - 253 of mouse GPR52 (Accession P0C5J4).
Accession (Uniprot) Number P0C5J4
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Standard Quality Control Of Each Lot Western blot analysis.
