Overview
Type: Synthetic peptide
Form: Lyophilized powder
GPR55 (extracellular) Blocking Peptide (#BLP-CR062) is the original antigen used for immunization during Anti-GPR55 (extracellular) Antibody (#ACR-062) generation. The blocking peptide binds and ‘blocks’ Anti-GPR55 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3), and mouse brain membranes (lanes 2 and 4):1, 2. Anti-GPR55 (extracellular) Antibody (#ACR-062), (1:500).
3, 4. Anti-GPR55 (extracellular) Antibody, preincubated with GPR55 (extracellular) Blocking Peptide (#BLP-CR062).
Western blot analysis of mouse BV-2 microglia cell line lysate:1. Anti-GPR55 (extracellular) Antibody (#ACR-062), (1:500).
2. Anti-GPR55 (extracellular) Antibody, preincubated with GPR55 (extracellular) Blocking Peptide (#BLP-CR062).
Expression of GPR55 in rat substantia nigra.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR55 (extracellular) Antibody (#ACR-062), followed by goat anti-rabbit-AlexaFluor-488. A. GPR55 immunoreactivity (green) appears in the substantia nigra pars compacta (SNC) in neurons (arrows). B. Pre-incubation of the antibody with GPR55 (extracellular) Blocking Peptide (BLP-CR062), suppressed staining. Cell nuclei are stained with DAPI (blue). SNR = substantia nigra pars reticulata.
Properties
Sequence
- (C)RNRFILD(S)RMKQGIS, corresponding to amino acid residues 261 - 275 of mouse GPR55 (Accession Q3UJF0)
Accession (Uniprot) Number Q3UJF0
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Standard Quality Control Of Each Lot Western blot analysis.
