Overview
Type: Synthetic peptide
Form: Lyophilized powder
GPR65/TDAG8 (extracellular) Blocking Peptide (#BLP-GR043) is the original antigen used for immunization during Anti-GPR65 (TDAG8) (extracellular) Antibody (#AGR-043) generation. The blocking peptide binds and ‘blocks’ Anti-GPR65/TDAG8 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human Jurkat T-cell leukemia (lanes 1 and 4), human THP-1 monocytic leukemia (lanes 2 and 5) and human HL-60 promyelocytic leukemia (lanes 3 and 6) cell line lysates:1-3. Anti-GPR65 (TDAG8) (extracellular) Antibody (#AGR-043), (1:200).
4-6. Anti-GPR65 (TDAG8) (extracellular) Antibody, preincubated with GPR65/TDAG8 (extracellular) Blocking Peptide (#BLP-GR043).
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-GPR65 (TDAG8) (extracellular) Antibody (#AGR-043), (1:200).
3,4. Anti-GPR65 (TDAG8) (extracellular) Antibody, preincubated with GPR65/TDAG8 (extracellular) Blocking Peptide (#BLP-GR043).
Expression of GPR65 in rat fornixImmunohistochemical staining of immersion-fixed, free floating rat frozen brain sections using Anti-GPR65 (TDAG8) (extracellular) Antibody (#AGR-043), (1:1200), followed by goat-anti-rabbit-AlexaFluor-488. GPR65 staining (green) appears in glial processes (vertical arrows) and in the side of fornix (FX) facing the ventricle (V, horizontal arrow). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)DYTWNKDNWTFSP, corresponding to amino acid residues 72 - 84 of human GPR65 (Accession Q8IYL9).
Accession (Uniprot) Number Q8IYL9
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
