Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)DYTWNKDNWTFSP, corresponding to amino acid residues 72 - 84 of human GPR65 (Accession Q8IYL9).
Accession (Uniprot) Number Q8IYL9
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human Jurkat T-cell leukemia (lanes 1 and 4), human THP-1 monocytic leukemia (lanes 2 and 5) and human HL-60 promyelocytic leukemia (lanes 3 and 6) cell line lysates:1-3. Anti-GPR65 (TDAG8) (extracellular) Antibody (#AGR-043), (1:200).
4-6. Anti-GPR65 (TDAG8) (extracellular) Antibody, preincubated with GPR65/TDAG8 (extracellular) Blocking Peptide (#BLP-GR043).
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-GPR65 (TDAG8) (extracellular) Antibody (#AGR-043), (1:200).
3,4. Anti-GPR65 (TDAG8) (extracellular) Antibody, preincubated with GPR65/TDAG8 (extracellular) Blocking Peptide (#BLP-GR043).- Rat brain sections (1:1200).
Last update: 02/01/2024
