Overview
Type: Synthetic peptide
Form: Lyophilized powder
GPR81/HCAR1 Blocking Peptide (#BLP-HR011) is the original antigen used for immunization during Anti-GPR81/HCAR1 Antibody (#AHR-011) generation. The blocking peptide binds and ‘blocks’ Anti-GPR81/HCAR1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney (lanes 1 and 3) and rat liver (lanes 2 and 4) lysates:1, 2. Anti-GPR81/HCAR1 Antibody (#AHR-011), (1:200).
3, 4. Anti-GPR81/HCAR1 Antibody, preincubated with GPR81/HCAR1 Blocking Peptide (#BLP-HR011) (#BLP-HR011).
Western blot analysis of mouse kidney lysate (lanes 1 and 3) and mouse liver membrane (lanes 2 and 4):1, 2. Anti-GPR81/HCAR1 Antibody (#AHR-011), (1:200).
3, 4. Anti-GPR81/HCAR1 Antibody, preincubated with GPR81/HCAR1 Blocking Peptide (#BLP-HR011) (#BLP-HR011).
Expression of HCAR1 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR81/HCAR1 Antibody (#AHR-011), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. HCAR1 immunoreactivity (green) appears in hippocampal blood vessels (horizontal arrows) and astrocytes (vertical arrows). B. Pre-incubation of the antibody with GPR81/HCAR1 Blocking Peptide (BLP-HR011), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of HCAR1 in mouse parietal cortex.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR81/HCAR1 Antibody (#AHR-011), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. HCAR1 immunoreactivity (green) appears in cortical blood vessels (horizontal arrows) and astrocytes (vertical arrows). B. Pre-incubation of the antibody with GPR81/HCAR1 Blocking Peptide (BLP-HR011), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- CSKSSIDGANRSQR, corresponding to amino acid residues 319-332 of mouse GPR81 (HCAR1) (Accession Q8C131).
Accession (Uniprot) Number Q8C131
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Standard Quality Control Of Each Lot Western blot analysis.
