Overview
Type: Synthetic peptide
Form: Lyophilized powder
GPR83 (extracellular) Blocking Peptide (#BLP-GR053) is the original antigen used for immunization during Anti-GPR83 (extracellular) Antibody (#AGR-053) generation. The blocking peptide binds and ‘blocks’ Anti-GPR83 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1, 2. Anti-GPR83 (extracellular) Antibody (#AGR-053), (1:400).
3, 4. Anti-GPR83 (extracellular) Antibody, preincubated with GPR83 (extracellular) Blocking Peptide (#BLP-GR053).
Western blot analysis of human Jurkat T-cell leukemia cell line lysate:1. Anti-GPR83 (extracellular) Antibody (#AGR-053), (1:400).
2. Anti-GPR83 (extracellular) Antibody, preincubated with GPR83 (extracellular) Blocking Peptide (#BLP-GR053).
Expression of GPR83 in mouse hippocampus and cortexImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR83 (extracellular) Antibody (#AGR-053), (1:3000), followed by goat anti-rabbit-AlexaFluor-488. A. GPR83 staining (green) in hippocampal dentate gyrus is detected in hilar interneurons (vertical arrows) and around cells of the granule layer (horizontal arrows). H = hilus of dentate gyrus. G = granule layer. B. GPR83 immunoreactivity (green) in parietal cortex is observed in neurons of the pyramidal layer (vertical arrows) and in their apical dendrites (horizontal arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RRYGAESQNPTVK, corresponding to amino acid residues 59 - 71 of mouse GPR83 (Accession P30731).
Accession (Uniprot) Number P30731
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
