Overview
Type: Synthetic peptide
Form: Lyophilized powder
GPR84 (extracellular) Blocking Peptide (#BLP-GR052) is the original antigen used for immunization during Anti-GPR84 (extracellular) Antibody (#AGR-052) generation. The blocking peptide binds and ‘blocks’ Anti-GPR84 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and rat spleen membranes (lanes 3 and 6):1-3. Anti-GPR84 (extracellular) Antibody (#AGR-052), (1:200).
4-6. Anti-GPR84 (extracellular) Antibody, preincubated with GPR84 (extracellular) Blocking Peptide (#BLP-GR052).
Western blot analysis of human THP-1 monocytic leukemia (lanes 1 and 4), human HL-60 promyelocytic leukemia (lanes 2 and 5) and human U-87 MG glioblastoma (lanes 3 and 6) cell line lysates:1-3. Anti-GPR84 (extracellular) Antibody (#AGR-052), (1:200).
4-6. Anti-GPR84 (extracellular) Antibody, preincubated with GPR84 (extracellular) Blocking Peptide (#BLP-GR052).
Expression of GPR84 in mouse fornix in a kainic acid-induced model of temporal lobe epilepsyImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR84 (extracellular) Antibody (#AGR-052), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. In untreated mouse sections GPR84 immunoreactivity (green) is detected in astrocyte profiles (arrows). B. GPR84 staining (green) in mouse sections obtained four days post kainate-induced seizures, shows an increase in number and size of astrocyte profiles stained for GPR84 (arrows). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- CT(S)SFDRIRGRPYTT, corresponding to amino acid residues 166-180 of human GPR84 (Accession Q9NQS5).
Accession (Uniprot) Number Q9NQS5
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
