Overview
Type: Synthetic peptide
Form: Lyophilized powder
GPR91/SUCNR1 (extracellular) Blocking Peptide (#BLP-SR090) is the original antigen used for immunization during Anti-GPR91 (SUCNR1) (extracellular) Antibody (#ASR-090) generation. The blocking peptide binds and ‘blocks’ Anti-GPR91/SUCNR1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney membrane (lanes 1 and 3), mouse kidney lysate (lanes 2 and 4), mouse TK-1 T-cell lymphoma (lanes 5 and 8), mouse J774 macrophage cell (lanes 6 and 9) and human Jurkat acute T cell leukemia (lanes 7 and 10) lysates:1,2,5,6,7. Anti-GPR91 (SUCNR1) (extracellular) Antibody (#ASR-090) (1:200).
3,4,8,9,10. Anti-GPR91 (SUCNR1) (extracellular) Antibody, preincubated with GPR91/SUCNR1 (extracellular) Blocking Peptide (#BLP-SR090).
Expression of GPR91 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR91 (SUCNR1) (extracellular) Antibody (#ASR-090), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. GPR91 immunoreactivity (green) appears in neuronal profiles in the pyramidal layer (P, vertical arrow) and the stratum oriens (SO, horizontal arrow). B. Pre-incubation of the antibody with GPR91 (SUCNR1) (extracellular) Blocking Peptide (BLP-SR090), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RNVRIASRLDSWPQG, corresponding to amino acid residues 248-262 of rat GPR91/Succinate Receptor 1 (Accession Q6IYF9).
Accession (Uniprot) Number Q6IYF9
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
