Overview
Type: Synthetic peptide
Form: Lyophilized powder
GRID1 (extracellular) Blocking Peptide (#BLP-GC038) is the original antigen used for immunization during Anti-GRID1 (extracellular) Antibody (#AGC-038) generation. The blocking peptide binds and ‘blocks’ Anti-GRID1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human CCF-STTG1 astrocytoma cell lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5) and rat brain lysate (lines 3 and 6):1-3. Anti-GRID1 (extracellular) Antibody (#AGC-038), (1:200).
4-6. Anti-GRID1 (extracellular) Antibody, preincubated with GRID1 (extracellular) Blocking Peptide (#BLP-GC038).
Expression of Glutamate receptor δ1 in rat cortex and medial septumImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-GRID1 (extracellular) Antibody (#AGC-038), (1:400). A. Staining in cortex. B. Staining in medial septum. In both regions, GluD1 expression (red) is detected in neurons (arrows). DAPI is used as the counterstain (blue).
Properties
Sequence
- (C)KDMRKLATWDSEK, corresponding to amino acid residues 407-419 of rat GluD1 (Accession Q62640).
Accession (Uniprot) Number Q62640
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
