Overview
Type: Synthetic peptide
Form: Lyophilized powder
GRID2 (extracellular) Blocking Peptide (#BLP-GC039) is the original antigen used for immunization during Anti-GRID2 (extracellular) Antibody (#AGC-039) generation. The blocking peptide binds and ‘blocks’ Anti-GRID2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human U87-MG glioblastoma cell lysate (lines 3 and 6):1-3. Anti-GRID2 (extracellular) Antibody (#AGC-039), (1:600).
4-6. Anti-GRID2 (extracellular) Antibody, preincubated with GRID2 (extracellular) Blocking Peptide (#BLP-GC039).- Mouse brain sections (1:200).
Pernice, H.F. et al. (2019) Int. J. Mol. Sci. 20, 1797.
Properties
Sequence
- (C)KMITTLFDTMRIE, corresponding to amino acid residues 206-218 of rat GluD2 (Accession Q63226).
Accession (Uniprot) Number Q63226
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
