Overview
Type: Synthetic peptide
Form: Lyophilized powder
GRIK1/GluK1 (extracellular) Blocking Peptide (#BLP-GC008) is the original antigen used for immunization during Anti-GRIK1 (GluK1) (extracellular) Antibody (#AGC-008) generation. The blocking peptide binds and ‘blocks’ Anti-GRIK1/GluK1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-GRIK1 (GluK1) (extracellular) Antibody (#AGC-008), (1:200).
2. Anti-GRIK1 (GluK1) (extracellular) Antibody, preincubated with GRIK1/GluK1 (extracellular) Blocking Peptide (#BLP-GC008).
Expression of GluK1 in rat cerebellumImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-GRIK1 (GluK1) (extracellular) Antibody (#AGC-008), (1:400), followed by donkey anti rabbit-Cy3 antibody. GluK1 staining (red) appears in the granule layer and in the baskets under Purkinje cells (arrows). Nuclei were labeled with DAPI and appear in blue.
Properties
Sequence
- (C)KASGEVSKHLYKVWKK, corresponding to amino acid residues 402-417 of rat kainate receptor GluK1 (Accession P22756).
Accession (Uniprot) Number P22756
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
