Overview
Type: Synthetic peptide
Form: Lyophilized powder
GRIK4/KA1 (extracellular) Blocking Peptide (#BLP-GC041) is the original antigen used for immunization during Anti-GRIK4 (KA1) (extracellular) Antibody (#AGC-041) generation. The blocking peptide binds and ‘blocks’ Anti-GRIK4/KA1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-GRIK4 (KA1) (extracellular) Antibody (#AGC-041), (1:400).
3,4. Anti-GRIK4 (KA1) (extracellular) Antibody, preincubated with GRIK4/KA1 (extracellular) Blocking Peptide (#BLP-GC041).
Expression of kainate receptor GluK4 in rat cerebellar deep nucleiImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-GRIK4 (KA1) (extracellular) Antibody (#AGC-041), (1:100). A. GluK4 staining (red) appears in neurons (arrows) of deep cerebellar nuclei. B. Cell nuclei in the same section are visualized with DAPI (blue). C. Merge of the two images.
Properties
Sequence
- (C)KNRINRAPERLGK, corresponding to amino acid residues 49-61 of rat kainate receptor GluK4 (Accession Q01812).
Accession (Uniprot) Number Q01812
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
