Certificate of Analysis

GTx1-15

Name: GTx1-15
Brand: Alomone Labs
SKU: STT-300

ω/β-Theraphotoxin-Ps2a, β-TRTX-Ps2a, Peptide E, β/ω-TRTX-Gr2a

A Blocker of Voltage-Gated Na⁺ and T-Type Ca²⁺ Channels

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Overview

Cat #: STT-300

Alternative Name ω/β-Theraphotoxin-Ps2a, β-TRTX-Ps2a, Peptide E, β/ω-TRTX-Gr2a

Lyophilized Powder yes

Origin Synthetic peptide

MW: 4074 Da

Purity: >98% (HPLC)

Effective concentration 200-500 nM.

Sequence DCLGFMRKCIPDNDKCCRPNLVCSRTHKWCKYVF-NH₂

Modifications Disulfide bonds between Cys²-Cys¹⁷, Cys⁹-Cys²³, Cys¹⁶-Cys³⁰, Phe³⁴ - C-terminal amidation.

Structure

Molecular formula C₁₇₆H₂₇₁N₅₃O₄₅S₇

Activity GTx1-15 inhibits rat Na_V1.3 and Na_V1.7 voltage-gated Na⁺ channels as well as T-type Ca²⁺ channels.

Accession number P0DJA9.

Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Solubility Soluble in water. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions Up to two weeks at 4°C or three months at -20°C.

Our bioassay

Alomone Labs GTx1-15 inhibits rat Na_V1.3 channels heterologously expressed in HEK-293 cells.
A. Dose-response of Na_V1.3 channel inhibition by GTx1-15 (#STT-300). The inhibition was measured in 3-5 cells for each dose. B. Example of superimposed current traces of rat Na_V1.3 channel activity before and during application of 0.3 µM of GTx1-15. Holding potential was -100 mV and currents were stimulated every 10 seconds by a voltage ramp of 40 msec from holding potential to +60 mV.

Scientific background

GTx1-15 is isolated from the Grammostola rosea (Phrixotrichus auratus) spider venom and is a synthetic version of the peptide^1,2.

GTx1-15 inhibits voltage-gated rat Na_V1.3 and Na_V1.7 Na⁺ channels, as well as T-type Ca²⁺ channels^1,2.

Voltage-gated Na⁺ channels (VGSC, Na_V) play a critical role in excitability of nociceptors (pain-sensing neurons).

The peripheral-specific Na⁺ channels Na_V1.7, Na_V1.8 and Na_V1.9 are particularly important in the pathophysiology of different pain syndromes and hence, thought to be potential targets for pain therapeutics^3-4. The expression and functional properties of Na_V channels in peripheral sensory neurons can be dynamically regulated following axonal injury or peripheral inflammation⁵.

GTx1-15 shows high homology to Phrixotoxin-3 (#STP-720) (76%) and Ceratotoxin-2 (#STC-100) (57%).

Target Na_V Na⁺ Channels, T-type Ca²⁺ channels

Peptide Content: 100%

Lyophilized Powder

For research purposes only, not for human use

Last Update: 07/05/2024

Specifications

Citations

Applications

Scientific Background

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