Name: Guinea pig Anti-Stargazin Antibody
Brand: Alomone Labs
SKU: ACC-012-GP

Overview

Cat #: ACC-012-GP

Alternative Name Ca_Vγ2, Voltage-dependent calcium channel gamma-2 subunit, CACNG2, TARP gamma-2, Transmembrane AMPAR regulatory protein gamma-2, Waggler

Lyophilized Powder yes

Type: Polyclonal

Host: Guinea pig

Reactivity: m, r

May also work in: h*

Immunogen

Peptide (C)RATDYLQASAITRIPS, corresponding to amino acid residues 213-228 of mouse Ca_Vγ2 (Accession O88602). Intracellular, C-terminus.

Accession (Uniprot) Number O88602

Gene ID 12300

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse – 14/15 amino acid residues identical; human – 12/15 amino acid residues identical.

RRID AB_2756616.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Guinea pig total IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: wb

May also work in: ic*, ifc*, ih*, ip*

Western blot

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
1,2. Guinea pig Anti-Stargazin Antibody (#ACC-012-GP), (1:500).
3,4. Guinea pig Anti-Stargazin Antibody, preincubated with Stargazin Blocking Peptide (#BLP-CC012).

References

Arikkath, J. et al. (2003) Curr. Opin. Neurobiol. 13, 298.
Chen, L. et al. (2000) Nature 408, 936.

Scientific background

The γ subunit protein is an auxiliary subunit of the Ca_V (voltage dependent calcium) channel multimer complex. γ1 was first identified in skeletal muscle as a part of the proteins complex purified as the DHP receptor. Eight γ subunit isoforms were identified in mammalian genomes and they share some sequence as well as structure homology¹.γ subunit contains four transmembrane helices, with intracellular N- and C- termini. Usually their association with the pore-forming α1 subunit results in reduced current amplitude.

Ca_Vγ2 is also known as Stargazin, and interacts and regulates the activity and trafficking of AMPA glutamate receptors².

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Guinea pig Anti-Stargazin Antibody (#ACC-012-GP, formerly AGP-069) is a highly specific antibody directed against an epitope of the mouse Ca_Vγ2 subunit. The antibody can be used in western blot analysis. It and has been designed to recognize Ca_Vγ2 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-Stargazin Antibody (#ACC-012) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 20/11/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Stargazin Blocking Peptide (#BLP-CC012) is the original antigen used for immunization during Anti-Stargazin Antibody (#ACC-012) generation. The blocking peptide binds and ‘blocks’ Anti-Stargazin primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Stargazin Blocking Peptide (#BLP-CC012)

Shipping and Ordering Information

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Products are lyophilized and ship at room temperature - FREE OF CHARGE.

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Guinea pig Anti-Stargazin Antibody