Overview
- Peptide CKQGFLSSNGQNHFK, corresponding to amino acid residues 58-72 of human EMR1 (Accession Q14246). Extracellular, N-terminus.
Western blot analysis of human promyelocytic leukemia (HL-60) (lanes 1 and 5), human Burkitt's lymphoma (Raji) B (lanes 2 and 6), mouse macrophage J774 (lanes 3 and 7) and rat basophilic leukemia (RBL) (lanes 4 and 8) cell lysates:1-4. Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051-GP), (1:200).
5-8. Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody, preincubated with EMR1/ADGRE1 (extracellular) Blocking Peptide (#BLP-ER051).
Western blot analysis of rat (lanes 9 and 11) and mouse (lanes 10 and 12) fat tissue lysate:9, 10. Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051-GP), (1:200).
11, 12. Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody, preincubated with EMR1/ADGRE1 (extracellular) Blocking Peptide (#BLP-ER051).
Western blot analysis of rat (lanes 13
and 15) and mouse (lanes 14 and 16) spleen lysate:13, 14. Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051-GP), (1:200).
15, 16. Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody, preincubated with EMR1/ADGRE1 (extracellular) Blocking Peptide (#BLP-ER051).
- Legrand, F. et al. (2014) J. Allergy Clin. Immunol. 133, 1439.
- McKnight, A.J. and Gordon, S. (1998) J. Leukoc. Biol. 63, 271.
- Araç, D. et al. (2012) EMBO J. 31, 1364.
Human epidermal growth factor (EGF)–like module containing mucin-like hormone receptor 1 (EMR1) is a surface receptor with unknown function that belongs to the adhesion family of the G protein-coupled receptors (GPCR). This receptor is encoded by the ADGRE1 and like all GPCRs, contains seven transmembrane domains, an intracellular C-terminus and an extracellular N-terminus. The NH2 domain has six EGF-like domains.
Adhesion GPCRs share a long N-terminal extracellular sequences comprising multiple domains, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties1,2.
EMR1 protein is a homolog of F4/80, a murine protein that is widely being used as a marker of murine macrophage populations1.
Human EMR1 is exclusively expressed on mature eosinophils in the blood and bone marrow and in nasal polyps. Thus, EMR1 is considered to be a potential therapeutic target for the treatment of eosinophilic disorders1,3.
Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051-GP, formerly AGP-214) raised in guinea pig, is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize ADGRE1 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.
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