Overview
- Peptide (C)ERNMHQMTDGLDK, corresponding to amino acids residues 194-206 of rat GAT-1 (Accession P23978). Extracellular 2nd loop.
GABA Transporter 1/GAT-1 (extracellular) Blocking Peptide (#BLP-GT001)
Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Guinea Pig Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody (#AGT-001-GP), (1:200).
3-4. Guinea Pig Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody, preincubated with GABA Transporter 1 (GAT-1) (extracellular) Blocking Peptide (#BLP-GT001).
Expression of GABA Transporter 1 in mouse cerebellum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Guinea Pig Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody (#AGT-001-GP), (1:300), followed by goat anti-guinea pig-AlexaFluor-594. A. GAT-1 immunoreactivity (red) appears in pinceau formations (arrows) around Purkinje cells (P). B. Pre-incubation of the antibody with GABA Transporter 1 (GAT-1) (extracellular) Blocking Peptide (#BLP-GT001), suppressed staining. Cell nuclei are stained with DAPI (blue). M = molecular layer, P = Purkinje cell layer.
Expression of GABA Transporter 1 in rat cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody (#AGT-001-GP), (1:300), followed by goat anti-guinea pig-AlexaFluor-594. A. GAT-1 immunoreactivity (red) appears in pinceau formations (arrows) around Purkinje cells (P). B. Pre-incubation of the antibody with GABA Transporter 1 (GAT-1) (extracellular) Blocking Peptide (#BLP-GT001), suppressed staining. Cell nuclei are stained with DAPI (blue). M = molecular layer, P = Purkinje cell layer.
- Gonzalez-Burgos, G. (2010) Adv. Pharmacol. 58, 175.
- Gether, U. et al. (2006) Trends Pharmacol. Sci. 27, 375.
- Meinild, A.K. et al. (2009) J. Biol. Chem. 284, 16226.
- Conti, F. et al. (2011) Front. Cell. Neurosci. 5, 2.
- Lewis, D.A. et al. (2005) Nat. Rev. Neurosci. 6, 312.
- Fueta, Y. et al. (2003) Neuroscience 118, 371.
- Frahm, C. et al. (2004) NeuroReport 15, 9.
γ-Aminobutiric acid (GABA) is the major inhibitory neurotransmitter in the mammalian central nervous system. Transmission via GABA can be halted by its reuptake through GABA transporters (GATs). These membrane proteins belong to the super family of slc6 genes which encode transporters responsible for the uptake of dopamine, serotonin, norepinephrine, glycine and GABA in a Na+-dependent manner1,2.
To date, four GATs have been identified: GAT-1, GAT-2, GAT-3 and BGT-1. Like all members of the slc6 family, they have twelve transmembrane domains and short intracellular N- and C-termini1. GATs couple the transport of one GABA molecule to 2 Na+ ions and one Cl- ion3. GAT-1 activity can be regulated at the level of its plasma membrane expression which is in part regulated by its trafficking via phosphorylation of residues in intracellular loops1.
GAT-1 expression is restricted to the central nervous system, specifically to axon terminals, in the neocortex and in some astrocytic processes4.
GAT-1 may be implicated in various psychological and neurological disorders such as schizophrenia, epilepsy and cerebral ischemia1,5-7.
Guinea Pig Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody (#AGT-001-GP) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GAT-1 from mouse, rat and human samples.
The antigen used to immunize guinea pigs is the same as Anti-GABA Transporter 1 (GAT-1) (extracellular) Antibody (#AGT-001) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.
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