Overview
Cat #:
ANR-016-GP
Host: Guinea Pig
Type: Polyclonal
Form: Lyophilized
Applications: IHC, WB
Reactivity: H, M, R
Alternative Name Axonal membrane protein GAP-43, Growth-associated protein 43, Neuromodulin, Neural phosphoprotein B-50
Accession (Uniprot) Number P07936
Gene ID 29423
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
For research purposes only, not for human use
Specifications
Properties
Immunogen
- Peptide (C)KPKESARQDEGKED, corresponding to amino acid residues 205-218 of rat GAP43 (Accession P07936). C-terminus.
Homology Mouse – identical; human – 13 out of 14 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Guinea pig total IgG
Standard quality control of each lot Western blot analysis.
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
GAP43 Blocking Peptide (#BLP-NR016)
GAP43 Blocking Peptide (#BLP-NR016)
Scientific Background
Scientific Background
Scientific background
Growth associated protein 43 (GAP43) is a neuron specific phosphoprotein that plays an important role in the process of nerve growth, development, regeneration and axonal remodeling. GAP43 belongs to the calmodulin-binding protein family and is highly distributed in the presynaptic membrane1.
GAP43 is expressed in developing neurons, especially in the growth cone. In mature neurons, GAP43 is detected in regenerating nerves. In brain injury, neurons exhibit sprouting of the lateral branches and responsive regeneration of the axon accompanied by increased expression of GAP43. GAP43 expression is used as an important tool for evaluating axon injury and the regenerative response in the mature central nervous system (CNS)1,2.
Last Update: 25/03/2025
