Name: Guinea pig Anti-KCNQ1 Antibody
Brand: Alomone Labs
SKU: APC-022-GP

Overview

Cat #: APC-022-GP

Alternative Name K_V7.1, Voltage-gated potassium channel QKT subfamily member 1, K_VLQT1

Lyophilized Powder yes

Type: Polyclonal

Host: Guinea Pig

Reactivity: h, m, r

Immunogen

Peptide (C)TYEQLTVPRRGPDEGS, corresponding to amino acid residues 661-676 of human KCNQ1 (Accession P51787). Intracellular, C-terminus.

Accession (Uniprot) Number P51787

Gene ID 3784

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, mouse - 14/16 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Guinea pig total IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Western blot analysis of rat heart membrane (lanes 1 and 3), rat spleen lysate (lanes 2 and 4), mouse heart membrane (lanes 5 and 6) and human Colo205 cell line lysate (lanes 7 and 8):
1,2,5,7. Guinea pig Anti-KCNQ1 Antibody (#APC-022-GP), (1:200).
3,4,6,8. Guinea pig Anti-KCNQ1 Antibody, preincubated with KCNQ1 Blocking Peptide (#BLP-PC022).

Immunohistochemistry

Expression of KCNQ1 in mouse heart
Immunohistochemical staining of mouse heart frozen section using Guinea pig Anti-KCNQ1 Antibody (#APC-022-GP), (1:200). A. KCNQ1 staining (red) appears in muscle fiber membranes (arrows). B. Nuclei are stained using DAPI counterstain (blue). C. Merged image of panels A and B.

References

Wang, Q. et al. (1996) Nat. Genet. 12, 17.
Grahammer, F. et al. (2001) Gastroenterology 120, 1363.
Mall, M. et al. (1998) Am. J. Physiol. 275, G1274.

Scientific background

KCNQ1 (K_V7.1) is part of a voltage-gated K⁺ family that includes 5 other members (KCNQ1-5).

The channel has been intensively studied since it was found that together with MinK (Isk) it is responsible for the cardiac I_ks current that controls the duration of the action potential of the human heart. Indeed, mutations in either K_V7.1 or MinK can be responsible for long QT syndrome, a cardiac disorder that causes arrhythmias and sudden death^1-3.

Although K_V7.1 expression and function have been mainly investigated in relation with cardiac function the channel is widely distributed in epithelial tissues where it is involved in physiological functions such as the regulation of acid secretion in the stomach and Cl^- secretion into the colon^1-3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Guinea pig Anti-KCNQ1 Antibody (#APC-022-GP, formerly AGP-050), raised in guinea pigs, is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize K_V7.1 channel from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-KCNQ1 Antibody (#APC-022) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 20/11/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

KCNQ1 Blocking Peptide (#BLP-PC022) is the original antigen used for immunization during Anti-KCNQ1 Antibody (#APC-022) generation. The blocking peptide binds and ‘blocks’ Anti-KCNQ1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

KCNQ1 Blocking Peptide (#BLP-PC022)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

Reagent Rewards

Not sure whether the product fits your experimental setup?

Try it first!

Alomone Guarantee

We offer 100% guarantee on all our products. If our products do not perform as stated, please contact our technical support staff.

Alomone Guarantee information

Navigation

Order Summary

Guinea pig Anti-KCNQ1 Antibody