Name: Guinea pig Anti-KCNQ2 Antibody
Brand: Alomone Labs
SKU: APC-050-GP

Overview

Cat #: APC-050-GP

Alternative Name K_V7.2, Voltage-gated potassium channel QKT subfamily member 2, KvLQT2

Lyophilized Powder yes

Type: Polyclonal

Host: Guinea Pig

Reactivity: h, m, r

Immunogen

Peptide (C)RGPTITDKDRTKGPAE, corresponding to amino acid residues 578-593 of rat KCNQ2 (Accession O88943). Intracellular, C-terminus.

Accession (Uniprot) Number O88943

Gene ID 170848

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - identical; human - 15/16 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Guinea pig total IgG.

Specificity The antibody is specific for KCNQ2. It does not cross-react with KCNQ3 or other QKT proteins. There are at least 9 recognized splice variants of rat KCNQ2. This antibody recognizes all except splice variants B and G.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Western blot analysis of rat brain (lanes 1 and 2), mouse brain (lanes 3 and 4), human KCNQ2 transfected HEK-293 cells (lanes 5 and 7) and human brain neuroblastoma SH-SY5Y cell line (lanes 6 and 8) lysates:
1,3,5,6. Guinea pig Anti-KCNQ2 Antibody (#APC-050-GP), (1:200).
2,4,7,8. Guinea pig Anti-KCNQ2 Antibody, preincubated with KCNQ2 Blocking Peptide (#BLP-PC050).

Immunohistochemistry

Expression of KCNQ2 in rat brain
Immunohistochemical staining of free-floating frozen rat hippocampal CA1 region sections using Guinea pig Anti-KCNQ2 Antibody (#APC-050-GP), (1:200). A. KCNQ2 staining (red) appears in the pyramidal layer (P). B. Nuclei are stained using DAPI as the counterstain (blue).

References

Kullmann, D.M. (2002) J. Mol. Brain 125, 1117.
Robbins, J. (2001) Pharmacol. Ther. 90, 1.
Wang, H.S. et al. (1998) Science 282, 1890.

Scientific background

The KCNQ family of voltage-gated K⁺ channels includes 5 known members: KCNQ1 to KCNQ5. Structurally, the KCNQ family belongs to the six transmembrane domain category of K⁺ channels.

KCNQ family members can form either homo- or heteromultimeric channels with different functional outcomes. For example KCNQ2 and KCNQ3 heteromultimers give rise to a much larger channel current than when either protein is expressed alone, probably due to enhanced plasma membrane expression of the combined channel. Indeed, KCNQ2/KCNQ3 heteromultimers are believed to be the molecular correlates of the so-called M current. This current is a K⁺ neuronal current that is strongly inhibited by the activation of the M₁ subtype of the muscarinic acetylcholine receptor.

Mutations in either KCNQ2 or KCNQ3 are associated with a form of epilepsy known as Benign familial neonatal convulsions (BNFC).

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Guinea pig Anti-KCNQ2 Antibody (#APC-050-GP, formerly AGP-065), raised in guinea pigs, is a highly specific antibody directed against an epitope of the rat K_V7.2 channel. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KCNQ2 from mouse, rat, and human samples.
The antigen used to immunize guinea pigs is the same as Anti-KCNQ2 Antibody (#APC-050) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 20/11/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

KCNQ2 Blocking Peptide (#BLP-PC050) is the original antigen used for immunization during Anti-KCNQ2 Antibody (#APC-050) generation. The blocking peptide binds and ‘blocks’ Anti-KCNQ2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

KCNQ2 Blocking Peptide (#BLP-PC050)

Shipping and Ordering Information

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Products are lyophilized and ship at room temperature - FREE OF CHARGE.

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Guinea pig Anti-KCNQ2 Antibody