Specificity The antibody is specific for KCNQ2. It does not cross-react with KCNQ3 or other QKT proteins. There are at least 9 recognized splice variants of rat KCNQ2. This antibody recognizes all except splice variants B and G.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
Rat and mouse brain, human KCNQ2 transfected HEK-293 cells and human brain neuroblastoma SH-SY5Y cell lines.
Western blot analysis of rat brain (lanes 1 and 2), mouse brain (lanes 3 and 4), human KCNQ2 transfected HEK-293 cells (lanes 5 and 7) and human brain neuroblastoma SH-SY5Y cell line (lanes 6 and 8) lysates:
The KCNQ family of voltage-gated K+ channels includes 5 known members: KCNQ1 to KCNQ5. Structurally, the KCNQ family belongs to the six transmembrane domain category of K+ channels.
KCNQ family members can form either homo- or heteromultimeric channels with different functional outcomes. For example KCNQ2 and KCNQ3 heteromultimers give rise to a much larger channel current than when either protein is expressed alone, probably due to enhanced plasma membrane expression of the combined channel. Indeed, KCNQ2/KCNQ3 heteromultimers are believed to be the molecular correlates of the so-called M current. This current is a K+ neuronal current that is strongly inhibited by the activation of the M1 subtype of the muscarinic acetylcholine receptor.
Mutations in either KCNQ2 or KCNQ3 are associated with a form of epilepsy known as Benign familial neonatal convulsions (BNFC).
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Western blot analysis of rat brain (lanes 1 and 2), mouse brain (lanes 3 and 4), human KCNQ2 transfected HEK-293 cells (lanes 5 and 7) and human brain neuroblastoma SH-SY5Y cell line (lanes 6 and 8) lysates:1,3,5,6. Guinea pig Anti-KCNQ2 Antibody (#APC-050-GP), (1:200).
