Name: Guinea Pig Anti-MRGPRD (GPCR TGR7) Antibody
Brand: Alomone Labs
SKU: AMR-061-GP

Overview

Cat #: AMR-061-GP

Alternative Name Mrgd, Mas-related G-protein coupled receptor member D, G-protein coupled receptor TGR7, β-Alanine receptor

Lyophilized Powder yes

Type: Polyclonal

Host: Guinea Pig

Reactivity: m, r

Immunogen

Peptide (C)KSHRLQESLGAVLGR, corresponding to amino acid residues 285-299 of rat MRGPRD (Accession Q7TN41). Intracellular, C-terminus.

Accession (Uniprot) Number Q7TN41

Gene ID 293648

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - identical. It will not recognize MRGPRD from human samples.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Guinea pig total IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 1 µg peptide per 1 µg antibody

Standard quality control of each lot Western blot analysis.

Applications: wb

May also work in: ic*, ih*, ip*

Western blot

Western blot analysis of rat dorsal root ganglion lysates:
1. Guinea Pig Anti-MRGPRD (GPCR TGR7) Antibody (#AMR-061-GP), (1:500).
2. Guinea Pig Anti-MRGPRD (GPCR TGR7) Antibody, preincubated with MRGPRD (GPCR TGR7) Blocking Peptide (BLP-MR061).
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) testis membranes:
1-2. Guinea Pig Anti-MRGPRD (GPCR TGR7) Antibody (#AMR-061-GP), (1:800).
3-4. Guinea Pig Anti-MRGPRD (GPCR TGR7) Antibody, preincubated with MRGPRD (GPCR TGR7) Blocking Peptide (BLP-MR061).

References

Uno, M. et al. (2012) J. Biomed. Biotechnol. 2012, 816159.
Wang, H. et al. (2009) J. Neurosci. 29, 13202.
Zylka, M.J. et al. (2003) Proc. Natl. Acad. Sci. USA. 100, 10043.
Rau, K.K. et al. (2009) J. Neurosci. 29, 8612.
Crozier, R.A. et al. (2007) J. Neurosci. 27, 4492.

Scientific background

Mas-related G-protein coupled receptor member D (MRGPRD) is expressed in the dorsal root ganglia, specifically in non-peptidergic, small-diameter, IB4+ C-fiber neurons that innervate the outer layers of the mammalian skin. They transmit polymodal nociceptive information from the skin epidermis to the substantia gelatinosa of the spinal cord.

MRGPRD receptor expressing neurons are essential for the full expression of mechanical nociception. Thus, MRGPRD represents an attractive therapeutic target for the development of agents for treating pain^1,2.

The Mrg family in mice is comprised of six single-copy genes: MrgD, MrgE, MrgF/RTA, MrgG, MrgH/GPR90, and MAS1. In addition, there are three large subfamilies: MrgA, MrgB,and MrgC³.

MRGPRD receptor is activated by β-alanine and is able to inhibit KCNQ/M-type potassium channels and to increase excitability of sensory neurons^4,5. MRGPRD has the ability to form heterodimers and is encoded by the TGR7 gene. This receptor is co-expressed with two nociceptors, including purinergic receptor (P2X3) and a vanilloid receptor (VR1)⁵.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Guinea Pig Anti-MRGPRD (GPCR TGR7) Antibody (#AMR-061-GP, formerly AGP-272) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot applications. It has been designed to recognize MRGPRD (GPCR TGR7) from rat and mouse samples. The antibody will not recognize MRGPRD from human samples.

The antigen used to immunize guinea pigs is the same as Anti-MRGPRD (GPCR TGR7) Antibody (#AMR-061) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 20/11/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

MRGPRD/GPCR TGR7 Blocking Peptide (#BLP-MR061) is the original antigen used for immunization during Anti-MRGPRD (GPCR TGR7) Antibody (#AMR-061) generation. The blocking peptide binds and ‘blocks’ Anti-MRGPRD/GPCR TGR7 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

MRGPRD/GPCR TGR7 Blocking Peptide (#BLP-MR061)