Overview
- Peptide (C)RNWKRPSEQLEAQH, corresponding to amino acid residues 256-269 of rat OX1R (Accession P56718). Intracellular, 3rd loop.
Orexin Receptor 1 Blocking Peptide (#BLP-OR001)
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Guinea Pig Anti-Orexin Receptor 1 Antibody (#AOR-001-GP), (1:200).
3-4. Guinea Pig Anti-Orexin Receptor 1 Antibody, preincubated with Orexin Receptor 1 Blocking Peptide (#BLP-OR001).
Expression of Orexin Receptor 1 in mouse locus coeruleus (LC)Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Guinea Pig Anti-Orexin Receptor 1 Antibody (#AOR-001-GP), (1:300), followed by goat anti-guinea pig-Alexa Fluor-594. A. OX1R immunoreactivity (red) appears in cells (vertical arrows) and in adjacent motor neurons of mesencephalic trigeminal nucleus (M5 horizontal arrows). B. Pre-incubation of the antibody with Orexin Receptor 1 Blocking Peptide (#BLP-OR001), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of Orexin Receptor 1 in rat dorsal root nucleus (DRN).Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-Orexin Receptor 1 Antibody (#AOR-001-GP), (1:300), followed by goat anti-guinea pig-Alexa Fluor-594. A. OX1R immunoreactivity (red) appears in DRN cells (arrows). B. Pre-incubation of the antibody with Orexin Receptor 1 Blocking Peptide (#BLP-OR001), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Sakurai, T. et al. (1998) Cell. 92, 573.
- Kukkonen, J.P. et al. (2002) Am. J. Physiol. Cell. Physiol. 283, C1567.
- Kirchgessner, A.L. (2002) Endocr. Rev. 23, 1.
- Sakurai, T. (2007) Nat. Rev. Neurosci. 8, 171.
Orexin Receptor 1 (OX1R) (also known as hypocretin receptor 1) is one of two receptors that recognize the peptide neurotransmitters orexin A and orexin B.1 Orexin A and B are 33 and 28 amino acids in length, respectively, and are derived from a common precursor termed prepro-orexin.
OX1R binds orexin A with greater affinity than orexin B (a one order of magnitude difference), while OX2R binds both ligands with similar affinities.2,3
Both OX1R and OX2R belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.
OX1R is thought to transmit signals through the Ga11 class of G proteins, resulting in the activation of phospholipase C with subsequent triggering of the phosphatidylinositol cascade and an influx of extracellular Ca2+, probably through transient receptor potential (TRP) channels.2,3
The physiological functions of the orexin system (OX1R, OX2R, and their ligands) have been a matter of intense research in the last few years.
OX1R is expressed in both the central nervous system and peripheral locations such as gastrointestinal tissues, pancreas, and testis.2 It appears to be involved in the regulation of feeding behavior in rodents since an OX1R antagonist is able to inhibit baseline feeding.2,3
The orexin system has been shown to be involved in the regulation of sleep and wakefulness states and OX1R knockout mice show defects in the regulation of rapid eye movement (REM) sleep, among other phenotypic alterations.4 In addition, the orexin system is involved in regulating autonomic functions such as blood pressure and heart rate, as well as in mechanisms that regulate the reward response in the brain.4
Guinea Pig Anti-Orexin Receptor 1 Antibody (#AOR-001-GP) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Orexin Receptor 1 from mouse, rat and human samples.
The antigen used to immunize guinea pigs is the same as Anti-Orexin Receptor 1 Antibody (#AOR-001) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.
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