Overview
- Peptide CTAENTLFYVKES, corresponding to amino acid residues 270-282 of human P2RY12 (Accession Q9H244). 3rd extracellular loop.
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1,2. Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020-GP), (1:400).
3,4. Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody, preincubated with P2Y12 Receptor (extracellular) Blocking Peptide (#BLP-PR020).
Western blot analysis of rat basophilic leukemia (RBL) cell line lysate (lanes 5 and 7) and human brain glioblastoma (U-87 MG) cell line lysate (lanes 6 and 8):5,6. Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020-GP), (1:400).
7,8. Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody, preincubated with P2Y12 Receptor (extracellular) Blocking Peptide (#BLP-PR020).
Expression of P2Y12 in rat parietal cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020-GP), (1:300), followed by goat anti-guinea pig-AlexaFluor-594. A. P2Y12 immunoreactivity (red) appears in axonal processes (arrows). B. Pre-incubation of the antibody with P2Y12 Receptor (extracellular) Blocking Peptide (BLP-PR020), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of P2RY12 in human glioblastoma (U-87 MG) cellsCell surface detection of P2RY12 in intact living U-87 MG cells. A. Extracellular staining of cells using Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020-GP), (1:100) followed by goat anti-guinea pig-AlexaFluor-594 secondary antibody (red). DAPI was used as the counterstain (blue). B. Live view of the cells merged with P2Y12 staining.
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- Abbracchio, M.P. et al. (2003) Trends Pharmacol. Sci. 24, 52.
- Nicholas, R.A. (2001) Mol. Pharmacol. 60, 416.
The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDPA). Eight functional mammalian P2Y receptors have been described: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y141-3.
The P2Y12 receptor is co-expressed with P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca2+ upon activation.
The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting4. The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders4.
Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020-GP, formerly AGP-098) is a highly specific antibody directed against an epitope of the human P2RY12. The antibody can be used in western blot and live imaging applications. It and has been designed to recognize P2RY12 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.
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